Spore testing with biological indicators should be performed at least which frequency?

• A. At least once per day
• B. At least once per week
• C. At least once per month
• D. At least once per year

Answer: (B)

Biological indicator spore testing verifies that the sterilization cycle actually achieves the necessary kill, confirming the entire process—from load configuration to time and temperature—worked as intended. The minimum recommended frequency is once a week. This cadence provides regular verification without being impractical for routine practice, and it helps catch equipment or process issues before contaminated instruments are used. Testing more frequently (daily) can be ideal in high‑risk settings, but weekly is the standard minimum; testing less often (monthly or yearly) risks missing failures and compromising safety. Always perform the test with the appropriate spores, read the results after incubation, and take immediate corrective action if growth occurs.